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Recombinant Bla g 4 重組德國小蠊過敏原Bla g4

產品分類 > 過敏疾病檢測原料 > Recombinant Bla g 4 重組德國小蠊過敏原Bla g4

Recombinant Bla g 4 重組德國小蠊過敏原Bla g4

產品貨號：RP-BG4-1
促銷： ￥8720.00
價格： ￥8720.00
分享到：
250ug
美國Indoor
Allergen:rBla g 4 (Blattella germanica allergen 4)

Unit:250μg

Source:P. pastoris

Mol. Wt:23kD

Purification:Purified from Pichia pastoris culture by phenol sepharose and gel filtration chromatography. Purity >95% by SDS-PAGE.

Concentration:

See product insert.

Formulation:

Preservative and carrier-free in phosphate buffered saline, pH 7.4. Filtered through 0.22 μm filter.

Storage:Store at -20oC.

Notes:(1) rBla g 4 is produced in P. pastoris as a glycosylated allergen with MW slightly higher than that predicted for natural Bla g 4 (21kD). (2) Not tested for endotoxin.

Genbank:U40767

PDB:3EBk, 3EBW, X-ray crystal structures

Product Resources:Recombinant Bla g 4 Certificate of Analysis

Allergens are provided for research and commercial use in vitro:  not for human in vivo or therapeutic use.

References:


  1. Vailes LD, Kinter MT, Arruda LK, Chapman MD. High level expression of cockroach allergen, Bla g 4 in Pichia pastoris. J Allergy Clin Immunol 1998;101:274-80.

  2. Arruda LK, Vailes LD, Hayden ML, Benjamin DC, Chapman MD. Molecular cloning of cockroach allergen, Bla g 4, identifies ligand binding proteins (or calycins) as a cause of IgE antibody responses. J Biol Chem 1995;270:31196-210.

  3. Arruda LK, Vailes LD, Ferriani VPL, Santos ABR, Pomés A, Chapman MD. The role of cockroach allergens in asthma. J Allergy Clin Immunol 2001;107:419-28.

  4. Fan Y, Gore JC, Redding KO, Vailes LD, Chapman MD, Schal C. Tissue localization and regulation by juvenile hormone of human allergen Bla g 4 from the German cockroach, Blattella germanica (L.). Insect Mol Biol, 2005;14:45-53.

  5. Tan YW, Chan SL, Ong TC, Yit lY, Tiong YS, Chew FT, Sivaraman J, Mok YK. Structures of two major allergens, Bla g 4 and Per a 4, from cockroaches and their IgE binding epitopes. J Biol Chem, 2009;284:3148-3157.